Reduce amplification bias caused by variable miRNA abundance using automated PCR normalization for NEXTFLEX small RNA sequencing workflows.

PCR Biosystems, the UK-based specialists in DNA and RNA analysis, have launched a range of IsoFast™ Bst Polymerase reagents for rapid, robust and sensitive DNA and RNA amplification. Through its ...

In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.

The "capture" can be realized through the hybridization of fragmented DNA with baits complementary to regions of interest, either on a microarray chip or free in solution. In-solution hybridization ...