The principle of the siPCR technique applied for the detection of mutant KRAS alleles in pancreatic fluid is shown in Figure 1A. The target sequence is amplified by PCR. Because one of the primers is ...

Throughout our evaluation of mutation enrichment by COLD-PCR platforms, we have observed a trend for the mutation enrichment related to the initial abundance of the mutation or variant fraction. As ...

Study Shows How Melting Point Analysis Infers the Presence of and Identifies SNPs The melting temperature (T m) of a PCR product is dependent on both the length and sequence of the DNA. Melting curve ...