For ethical and safety reasons it is critical to develop easily implemented assays with high sensitivity and specificity for gene doping surveillance. Two nested quantitative real-time PCR (qPCR) ...

CRISPR is a technique that is revolutionizing biomedical research through high-precision genome editing. However, even though it allows the creation or correction of mutations consisting of a single ...

PCR or Polymerase Chain Reaction is a revolutionary method that is used to amplify specific segments of DNA or RNA. This method was developed by Kary Mullis in the 1980s. Kary Mullis received the ...

Researchers used the model organism Caenorhabditis elegans to optimize a new technique, leading to the development of the method called Nested CRISPR. This cloning-free method involves the insertion ...